منابع مشابه
Spin label studies on the flavoproteins lipoamide dehydrogenase and D-amino acid oxidase.
Maleimide spin label was covalently bound to sulfhydryl residues of D-amino acid oxidase and lipoamide dehydrogenase. Labeling of native D-amino acid oxidase resulted in a non-homogeneous EPR-spectrum, which consisted of 4 moles of spin label bound immobile to the enzyme (mol.-weight 90.000). A detailed analysis of the spectrum, the kinetics of the reaction of the spin label with the protein an...
متن کاملPreliminary Report of NAD+-Dependent Amino Acid Dehydrogenase Producing Bacteria Isolated from Soil
Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...
متن کاملKinetic and equilibrium studies on crystalline 1-glutamic acid dehydrogenase.
The spectrophotometric procedure for determining the specific activity of the enzyme and the preparation of reagents employed in the assay have been described previously (3). DPiV-Commercial preparations of DPN (60 per cent) were used in routine assays. For critical experiments, commercial DPN was further purified by several adsorptions on Dowex 1 formate columns by the method of Kornberg and P...
متن کاملStudies on Succinic Dehydrogenase
About 2 years ago the author reported that the activity of purified preparations of succinic dehydrogenase is enhanced by phosphate buffer (2). This enhancement was dependent upon the phosphate concentration and appeared to be relatively specific for phosphate, since other buffers, such as Tris,l imidazole, histidine, glycylglycine, and arsenate did not induce it (2, 3). Arsenate, in fact, inte...
متن کاملStudies on Succinate Dehydrogenase
1. Succinate dehydrogenase flavocoenzyme (“SD-flavin”), previously shown to be an SOLsubstituted riboflavin derivative containing a tertiary nitrogen homoconjugated to the flavin nucleus, was subjected to further hydrolysis and to reduction under acid conditions. Both conditions resulted in the liberation of 1 mole of histidine per mole of flavin. This proves histidine to be the covalent link b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1936
ISSN: 0306-3283
DOI: 10.1042/bj0301080